DESTO (Pakistan), the Defence Science and Technology Organization
Desto Records, a defunct American classical music record label based in New York active 1964-1982
Dr. Desto, a villain in the Hanna Barbera cartoon Samson & Goliath
Desto (Pty) Ltd, a company registered in South Africa in 1994, that provides Education, Training and Development and Consulting and Recruitment Services
Dual Stage To Orbit/Double Stage To Orbit, a spacecraft in which two distinct stages provide propulsion consecutively in order to achieve orbital velocity